(Fighting against New Coronary Pneumonia) Visiting Wuhan City No.1 Hospital nucleic acid inspector to arrest "poison"

　　China News Service, Wuhan, May 14th (Qiao Lingling, Dai Yuming) The Ministry of Human Resources and Social Security has recently announced 10 new occupations, among which nucleic acid detectors are listed. The reporter came to the PCR laboratory of the First Hospital of Wuhan City on the 14th to visit the "post-90s" nucleic acid inspector He Jing's daily anti-drug work.

The picture shows the nucleic acid inspector He Jing taking the inactivated sample into the PCR laboratory. Dai Yumi

　　He Jing, 27, graduated from Hubei University of Traditional Chinese Medicine with a major in medical laboratory. After obtaining the inspection technician certificate, he entered the blood transfusion department of Wuhan First Hospital. During his work, he studied for a master's degree in immunology at Tongji Medical College of Huazhong University of Science and Technology. Usually, He Jing and colleagues are mainly engaged in the detection of cross-matching blood, infectious diseases, blood coagulation, etc. and clinical blood transfusion treatment. Since the epidemic, her blood transfusion department has successively undertaken the detection of new coronavirus antibodies and nucleic acids. A nucleic acid inspector.

Figure why scan the barcode on the sample to number the samples one by one. Dai Yumi

　　PCR laboratory is also called gene amplification laboratory. PCR is the abbreviation of polymerase chain reaction. It is a molecular biology technology used to amplify specific DNA fragments. It can be regarded as special DNA replication in vitro. Through the DNA gene tracking system, it can master the detection of specific nucleic acid fragments in samples. The content.

Figure why statically transfer the numbered samples from the transfer window to the nucleic acid extraction room. Dai Yumi

　　He Jing introduced that the new coronavirus is a highly pathogenic pathogenic microorganism, and all the test samples are concentrated in the laboratory, just like a high-risk "nuclear bomb." As a nucleic acid inspector, the first step of the work is to strictly wear three-level protective equipment as required, three layers of latex gloves, N95 masks, protective glasses, disposable shoe covers, one-piece protective clothing, isolation clothing, etc. less.

The picture shows two nucleic acid detectors performing nucleic acid extraction in a biological safety cabinet. Dai Yumi

　　The whole process of nucleic acid detection of New Coronavirus is divided into four major steps: sample (usually pharyngeal swab) collection, transport, detection and experimental data reporting. He Jing is engaged in the most professional nucleic acid extraction and gene expansion in this ring. increase.

Why does the picture look at the nucleic acid detection results? Dai Yumi

　　Nucleic acid extraction must be performed in a biological safety cabinet. He Jing and his colleagues work in groups of two. After one person prepares the reagents, the other person transfers the inactivated samples into a special reaction tube with a sample gun. When the specimen is transferred into the reaction tube, try not to splash it to prevent cross infection between the specimens. Then the gene amplification was carried out by a fluorescent quantitative PCR instrument, and one instrument can remove 94 negative positive controls in 96 wells at a time and can measure 94 people. One full operation of the instrument is 45 cycles, about 1 hour and 45 minutes.

Figure why use a sample gun to transfer the sample into a special reaction tube to extract nucleic acid. Dai Yumi

　　The last part is the reporting of experimental data. Both suspicious and positive samples will be reviewed, and the results suggest that the nucleic acids that were not successfully extracted also need to be reviewed. "In order to ensure the accuracy of the test, the laboratory will uniquely number each test specimen from the time the specimen enters to the result. During the period, a total of 3 confirmations and verifications are required." He Jing said. (Finish)