The game at the RZD Arena stadium ended with a score of 3:1 in favor of the wards of Slavisa Jokanovic.
Thus, for the first time since 2010, the red-greens lost three home matches in a row in the RPL.
The club has also suffered five defeats in their last six games.
Dynamo moved up to sixth place in the standings with 23 points.
Lokomotiv is 14th with 12 points.
Earlier, Lokomotiv coach Andrey Fedorov told what the team lacked in the match against Dynamo.