China News Service, Chengdu, May 29 (He Shaoqing and Zhang Zhe) The reporter learned from Sichuan Agricultural University on the 29th that the team of State Key Laboratory of Sichuan Agricultural University and Rice Research Institute Professor Li Shigui and Qin Peng teamed up with Liang from the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences. The analysis of Chengzhi's team revealed "hidden" mutations in the rice genome.
This research result was titled "Pan-genomic analysis based on 33 rice genetic diversity materials revealing "hidden" genomic variation", which was published online by the internationally renowned academic journal "Cell" late at night on the 28th Beijing time.
The Sichuan Agricultural University and the Chinese Academy of Sciences team selected 33 highly representative rice materials and used the latest third-generation gene sequencing technology to perform long-segment sequencing, high-quality genome assembly and gene annotation on 31 of them.
Combining the reported reference genomes of Nipponbare and Shuhui 498 materials, after systematic comparative analysis, a total of 171072 gene structural variants and 22549 gene copy number variants were identified.
These genomic variants cannot be identified by traditional methods, and most of them have not been discovered in previous studies, but they play an important role in the regulation of important agronomic traits.
For example, an early maturity site (qDTH7-3) in the famous high-quality rice variety "Koshihikari" is likely to be caused by two copies of OsMADS18 gene in "Koshihikari", leading to an increase in gene expression and an early maturity phenotype.
In the course of this research, Chinese researchers also constructed the graphic genome of rice for the first time, which is the most complete pan-genome based on graphic structure in rice so far.
In order to facilitate the use of relevant data by researchers, they also set up a website to facilitate the use of genome sequences and search for genetic variation and other content to promote the application of rice functional genomics and breeding.
Li Shigui said that this research has opened the door to structural variation research, will help accelerate rice functional genomics and molecular design breeding research, and provide basic support for the breeding of new high-yield, high-quality, green and safe rice varieties.
Next, the team will apply the research results to applied basic research and breeding work, and strive to cultivate more rice varieties with high yield, good quality and strong resistance.
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