Mobile working is the future for many companies, but anathema to Elon Musk.
The new Twitter boss announced the return to the previous system in his first email to the workforce, the Bloomberg news agency reported on Thursday.
He expects employees to spend at least 40 hours a week in the office.
Twitter initially did not respond to a request from the Reuters news agency.
Musk had previously made similar announcements at his other companies such as the space company Space-X or Tesla.
He told the employees of the electric car manufacturer in an email in the summer: "If you don't show up, we will assume that you have resigned."
Musk acquired Twitter for $44 billion two weeks ago.
He is currently busy with the restructuring of the company.
After firing around half of the approximately 7,000 employees and many high-ranking managers, he is currently gathering a new circle of management personnel around him.
Restructuring of the company and management
Veteran Twitter executive Yoel Roth is said to be responsible for trust and security issues, according to the Bloomberg financial agency.
Over the past two weeks, this space has come under pressure from advertisers and activists who fear a kind of Wild West of opinion and misinformation on Twitter.
So far, nothing has changed in the moderation of content on Twitter, as Musk also emphasized.
Benham Rezaei becomes responsible for product development and programming.
Its division is also undergoing extensive restructuring.
According to information from Bloomberg, marketing and sales will be in the area of Robin Wheeler in the future.
She is currently busy assuaging advertisers' concerns.