Jacobs won in 9 sec 95 (0.1 m/s wind) ahead of two Britons: defending champion Zharnel Hughes (9.99) and Jeremiah Azu (10.13).
Frenchman Mouhamadou Fall took 5th place in 10 sec 17.
Despite an average start, Jacobs ended up proving to be the most powerful.
Straps in the colors of Italy on the left leg were a reminder that the sprinter had had his summer season ruined by injuries.
Highly anticipated, he withdrew before the semi-finals of the World Championships in Eugene last month due to a contracture of the adductor magnus of the right thigh this time.
Jacobs had been crowned Olympic champion to everyone's surprise a year ago.
He was then titled in the 60m at the Indoor Worlds in March in Belgrade.
Mouhamadou Fall (30) took 5th place in his first individual international final.
The three-time French 100m champion is under threat of suspension for three breaches in less than a year of his anti-doping location obligations.
He is thus only engaged in individual in Bavaria (100 and 200 m).
© 2022 AFP