It was local time on Saturday night when police were alerted that screams were heard from inside a shipping container that was abandoned between the cities of Nueva Concepción and Cocales in western Guatemala.
When the container was broken into, 126 people were found - men, women and children - who, according to police theory, had been abandoned by human traffickers.
- We heard crying and knocking from inside the container.
When we opened the doors, we found 126 undocumented people, says a police spokesperson according to the BBC.
About 100 of the migrants found are said to come from disaster-stricken Haiti, where thousands of residents have fled the country in recent months due to poverty, natural disasters and political instability.
Others are believed to be residents of Nepal and Gahna, writes the BBC.
Second discovery in two days
According to Alejandra Mena, a spokeswoman for the Guatemalan Migration Board, the migrants had traveled to Honduras to try to travel to the United States via Mexico.
All received medical care on site before being taken to a temporary detention center for handover to authorities.
The discovery comes shortly after Mexican soldiers on Thursday found 652 migrants, including 350 children, in three refrigerated trucks near the US border, which sheds new light on the current situation of migrants in the area.
In the last month alone, 13,000 migrants, most of whom are Haitian citizens, have gathered under a bridge on the Rio Grande on the Mexican-Texas border in hopes of obtaining a temporary residence permit in the United States.
At the same time, more than 7,500 people have been deported to Haiti.
"Our borders are not open and people should not try to make the dangerous journey," Marsha Espinosa of the US Department of Homeland Security told the BBC.