England coach Gareth Southgate said midfielder Jordan Henderson was out between the two halves of the match against Belgium.
Due to a leg injury, more doubts are surrounding the player's readiness to play with injury-plagued Liverpool next week.
Harry Winks played instead of Henderson at the start of the second half, during a match that ended with England losing with two unanswered goals in the European Nations League, on Sunday, to end the team's hopes of reaching the finals of the competition.
"Jordan suffered an injury at half-time, and he felt he could not continue," Southgate told reporters, adding that he was "not sure if the player had a hamstring injury."
Southgate confirmed that the Liverpool captain would undergo tests to determine his condition.
Liverpool is already suffering from the absence of a group of key players, as defenders Virgil van Dyck and Joe Gomez are absent for a long time after undergoing two surgeries, while Trent Alexander-Arnold is out of the England squad;
Because of suffering an injury.
And Liverpool's troubles increased after doubts arose about Andy Robertson's condition;
Due to a hamstring injury, midfield duo Thiago Alcantara and Alex Oxlade Chamberlain recover from two knee injuries.
Mohamed Salah appears to be a candidate for missing Leicester City next week, in light of his isolation in Egypt after contracting Covid-19.
Southgate said Manchester City's Raheem Sterling will miss the last game in the group against Ireland.
Due to a leg injury, he will be ready to play for his club next week.
Southgate, 50, revealed that he had contracted the Corona virus last month.
He said, "I was infected with the virus, but I was lucky ... it was not dangerous, as is the case for many in our country, it was not good, but now I am fine."