34-year-old Lewandowski has scored hat-tricks for Borussia Dortmund, Bayern Munich and now Barcelona.
In the past, Cristiano Ronaldo, Filippo Inzaghi and Neymar have scored hat-tricks for two different clubs.
Afterwards, he was praised by the opposing coach.
- Lewandowski showed his class tonight.
His finishing is phenomenal and he always chooses the perfect position in the box.
He is rightly regarded as one of the world's best strikers, says Viktoria Plzen's Michal Bilek, according to Uefa.
Lewandowski has started the season as a "Haaland light" and scored eight goals for his new club in five games after joining from Bayern Munich last summer.
Unlike last season, Barcelona are a team in harmony and the 77,000 at Camp Nou enjoyed tonight's display.
Czech side Viktoria Plzen came to Catalonia on a 34-game unbeaten run in all competitions (29 wins and five draws).
Tonight they weren't even close to getting anything.
Kulusevski substituted for Tottenham
Dejan Kulusevski entered the field after 61 minutes as Tottenham hosted Marseille.
With barely a quarter to go, Richarlison got a hole in the French, who played almost the entire second half with one man less.
The Brazilian also made it 2-0 shortly afterwards.