The English Premier League clubs will be fined £25 million (about 29 million euros) and thirty points deducted if they make another attempt to set up a Super League.
The league management announced this on Wednesday.
The Premier League's decision follows the intended participation of Manchester City, Manchester United, Chelsea, Liverpool, Arsenal and Tottenham Hotspur in the closed European elite competition.
Under heavy pressure from supporters, unions, sponsors and British Prime Minister Boris Johnson, they withdrew from the project at the end of April.
Nevertheless, the six clubs will be fined 22 million pounds (more than 25 million euros) for setting up the Super League, which had to replace the current Champions League.
The money will go to new opportunities for supporters and community projects.
"The six clubs involved in the creation of the Super League have today once again acknowledged that their actions were a mistake," the Premier League said in a statement after consulting with all 20 clubs.
"They have reaffirmed their commitment to the Premier League and the future of English football."
It was previously announced that new owners in the Premier League must sign a statement that they will not participate in the Super League with their club.
An investigation by the English Football Association FA is currently ongoing into the role of English clubs in the creation of the competition.
Only FC Barcelona, Real Madrid and Juventus are sticking to the plans for the Super League. The European football association UEFA launched disciplinary proceedings against the three clubs at the end of last month. It is not clear what punishment the clubs risk. At the same time, a European judge is investigating whether UEFA can block a Super League.