The match, which took place at the Volgograd Arena, ended in a 2-0 victory for the guests.
Grzegorz Krykhovyak and Rifat Zhamaletdinov scored goals.
Lokomotiv ranks third in the RPL standings with 43 points.
Rotor is 13th with 18 points.
Earlier, Selyuk described Chorluka's upcoming departure from Lokomotiv with words from the song.