The Dutchman, in his best attempt, covered the lap in 1 minute 37.378 seconds.
Finn Valtteri Bottas from Mercedes was the second, and the third - Frenchman Esteban Ocon from Renault.
Russian racer Alpha Tauri Daniil Kvyat took eighth place in the final protocol of the session.
Earlier it was reported that the seven-time Formula 1 champion Briton Lewis Hamilton will perform at the Abu Dhabi Grand Prix.