Bird flu has been diagnosed at a poultry farm in Altforst, Gelderland, the Ministry of Agriculture said in a press release on Thursday.
The animals are infected with the highly pathogenic H5 variant that infects humans.
Bird flu was recently diagnosed in two dead swans in the Netherlands.
It concerns a company where parent animals of broilers are kept.
They will all be cleared because of the infections.
The ministry is investigating nine other businesses in the area.
For 25 others within a radius of 10 kilometers, a ban applies to the transport of all animals, manure and animal products, among other things.
Since last Friday, a house requirement applies to all commercial poultry farms in the Netherlands, after the discovery of the infected mute swans in Kockengen in Utrecht.
The ministry has announced additional measures for hobby bird owners to ensure that their animals do not come into contact with waterfowl.
There is also a ban on the display of ornamental poultry and water birds.
The two mute swans in Utrecht have probably become infected because migratory birds from Russia have taken the bird flu virus with them.
Birds with bird flu have been reported several times in Russia.
The H5 bird flu virus can also infect humans.
However, that doesn't happen often.
Since 2003, 861 human infections and 455 deaths have been reported to the WHO health organization.
Different types of bird flu
Bird flu or avian influenza comes in many different variants.
There are mild, low pathogenic variants (LPAI) and dangerous, highly contagious highly pathogenic versions (HPAI).
(Classic) avian influenza occurs when many birds become infected with a dangerous variant at the same time and die.
Birds can pass on the avian flu virus to each other through respiration, eye fluid and manure.
Chickens and turkeys are the most susceptible to contamination.
People and commercial transports can also spread the virus.
Travelers can, for example, already do this via a leftover bird droppings under their shoes.
Poultry sector was previously affected by avian flu
In 2003, the Dutch poultry sector was hit hard by H7N7 avian influenza.
Chickens, turkeys and ducks were killed at 1,349 poultry farms.
In total, 30.7 million farm and hobby animals were culled.
Bird flu of the H5N8 type was found in four places in 2014.
After these outbreaks, the then cabinet divided the country into four regions in order to contain the avian flu outbreak as much as possible.
The virus continued to appear regularly in the years that followed, but the infection could each time be limited to a few companies at most.