The Irish Rugby Union Federation has decided to comply with the latest guidelines from World Rugby.
She announced on Wednesday that transgender players will no longer be allowed to participate in matches for the coming season.
Only players registered as female at birth will be able to play matches.
Ireland notably joins the decision of English rugby union and the Rugby League, which last month banned transgender players from taking part in women's competitions for safety reasons.
“Recent research shows that there are physical differences between people whose sex was assigned as male or female at birth.
The strength, endurance and physical benefits brought on by male puberty are significant and continue even after testosterone is removed,” the Irish Rugby Football Union (IRFU) said in a statement.
Two players involved
“IRFU is fully aware that this is a sensitive and challenging area for those involved and the wider LGBT+ community and will continue to work with those affected, providing support to ensure their continued involvement in the game, ”added the instance.
The rule change will only affect two players registered in Ireland.
IRFU said it had contacted them to discuss "options for staying active" in the community, "such as non-contact forms of play, officiating and coaching".
“Ireland should not follow the UK's lead in trying to reduce the number of occasions in which trans women can safely exist.
Ireland can and must do better”, reacted Paula Fagan, director general of the LGBT movement in Ireland, asking the IRFU to “reconsider its decision”.
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