The meeting took place in Moscow at the Russian Railways Arena and ended with a score of 2: 0.
At the 22nd minute, François Camano brought the railroad workers ahead after a cross by Fedor Smolov.
In the 45th minute, the red and white were outnumbered after Ayrton was sent off for a foul of last resort on Rifat Zhemaletdinov, and then Smolov doubled the hosts' lead with a free-kick.
Lokomotiv scored 46 points and ranks third in the standings of the Russian championship.
Spartak is in second position with 47 points.
Earlier, the referee Igor Fedotov assessed the episode with an unassigned penalty on Spartak striker Quincy Promes in the match against Lokomotiv.