Kosovars Asllani and Sofia Jakobsson's Real Madrid lost and broke their five-match winning streak, losing the Madrid derby to Atletico Madrid 0-1.
Merel van Dongen gave Atletico the lead after just five minutes when she got the ball in the aftermath of a corner and put the ball in goal.
The Real players protested immediately and thought it was hands on but the goal remained.
In the 52nd minute, Kosovar Asllani talked to a yellow card and the Swede was then replaced in the 85th minute.
Despite just over six extra minutes and Real's best goal chance in the match coming in the final minutes, they did not manage to break the deadlock and Atletico took all three points.
Real Madrid is third in the Primera división femenina.