UEFA appointed Stéphanie Frappart on Monday as the first ever female referee in the Champions League.
The Frenchwoman will be in charge of Wednesday's match between Juventus and Dynamo Kyiv.
At the Juventus Stadium, Frappart is assisted by male linesmen and a male fourth official.
The 36-year-old referee has made a rapid career in recent years.
In April last year, Frappart already became the first female referee in the French Ligue 1. Later that year, she also refereed the European Super Cup between Liverpool and Chelsea (2-2, Liverpool won on penalties).
This season, she already played in three games in the Europa League.
In addition, Frappart was in charge of an international match for the men for the first time in September: Malta-Latvia (1-1).
She also regularly whistles matches in women's football.
The highlight last year was the World Cup final in Paris between the United States and the Orange (2-0).
Juventus already sure of wintering
The Italian champion Juventus is already certain of wintering in the Champions League.
Dynamo Kiev is still fighting with Ferencvaros for third place and with it a ticket for the Europa League.
The match between Juventus of defender Matthijs de Ligt and Dynamo Kiev starts on Wednesday at 9 p.m.
View the standings and the program in the Champions League