Anna van der Breggen also won the world title on the road on Saturday in Imola, Italy.
De Zwolse, who was also the strongest in the time trial last Thursday, crowned an impressive solo with the victory in Imola.
Van der Breggen jumped 41 kilometers from the finish on the fourth ascent of the Cima Gallisterna and crossed the line solo with a big lead.
Annemiek van Vleuten made the Dutch party complete by taking the silver at about a minute and a half from her compatriot.
The defending champion trumped Italian Elisa Longo Borghini in the sprint for second place.
Marianne Vos finished fourth.
The thirty-year-old Dutch rider is only the second rider to succeed in grabbing gold in both the road race and the time trial at one World Championship.
Only the Frenchwoman Jeannie Longo managed to achieve that in 1995.
For Van der Breggen it is her second world title on the road.
Two years ago she won the world title in the road race for the first time in Innsbruck.
She provides the fourth Dutch world title on the road in a row, after Chantal Blaak (2017), herself (2018) and Van Vleuten (2019) have triumphed over the past three years.
Leading group with Pieters gets little space
The road race route took the peloton 143 kilometers to and from the Imola circuit.
Five laps of almost 18 miles had to be completed, each of which involved climbing the Mazzolano and the Cima Gallisterna.
Only after 59 kilometers did the first leading group form.
Amy Pieters and eight other riders took a lead of about two minutes, but the nonet was caught again shortly before the fourth climb of the Cima Gallisterna.
On that 2.3-kilometer climb with a maximum gradient of 14 percent, Annemiek van Vleuten attacked from a favorite group of about thirty riders.
Subsequently, Van der Breggen took over the direction of her compatriot and ran off alone.
Van der Breggen quickly expands its lead
The brand new world champion in the time trial soon had a 45-second lead over a chasing group, which consisted of Van Vleuten, Elisa Longo Borghini, Cecilie Uttrup Ludwig and Elizabeth Deignan.
Van der Breggen continued to expand that difference and with another 15 kilometers she had a two-minute lead over the thinned peloton, which had caught the four pursuers again.
At the last passage of the Cima Gallisterna, Longo Borghini drove away from the peloton and a little later she was joined by Van Vleuten.
Van der Breggen's victory was no longer in danger and she soloed to her second world title on the road.