Kiki Bertens was injured on Wednesday to give up in the second round of the WTA tournament in Strasbourg.
The Dutch threw in the towel against Jelena Ostapenko in the third set.
28-year-old Bertens seemed on her way to a quick victory over the Latvian, but she dropped way back in the match.
At a score of 6-2, 4-6 and 2-4, the Westland called a doctor on the court because of ankle complaints and decided to give up.
It is not clear how serious her injury is.
Last week, Bertens made her official return to the clay tournament in Rome, but due to a lack of competition rhythm, she remained far from her usual level in the Italian capital.
She went directly down in her first match against the Slovenian Polona Hercog.
Because of her early elimination in Rome, Bertens decided to accept a wild card for the tournament in Strasbourg, so that she can gain more rhythm and confidence for Roland Garros.
Her assignment is logically bad news in view of the Grand Slam tournament in Paris, which starts on Sunday.
Just like Arantxa Rus, Bertens is directly qualified for Roland Garros.
The leader of Dutch tennis, who reached the semi-finals in Paris in 2016, had to give up sick in the second round last year.