The runners will run along Rocksjön, Munksjön and Lake Vättern.
At the end of the course, twelve water and refill stations will be located to fill up with new energy such as fruit, energy drink and pickles.
Parts of Jönköping are closed for the benefit of the race.
Including the Munksjö bridge, the parking lot at the cultural center Spira, Stenhuggargatan and the underpass at Östra Storgatan.
According to the municipality, parts of John Erikssongatan and roads around Ekhagen center will also be closed.