The figure was published by the Robert Koch Institute for Public Health Surveillance (RKI).
The latest record stood at 197.6, broken on December 22, 2020.
While many more people are now vaccinated, the vaccination rate has nevertheless stagnated below 70%.
The authorities have called on the population to mobilize in recent days.
"For the unvaccinated, the risk is high that they will be infected in the coming months," RKI chief Lothar Wieler warned on Wednesday.
In eastern Saxony, where the incidence rate is more than double the national average at 491.3, unvaccinated people are subject to further restrictions on Monday.
Covid-19: the incidence in Germany Kenan AUGEARD AFP
Access to indoor restaurants and indoor events will be limited to people who have been fully vaccinated or who can show proof of recovery from Covid-19.
These new rules are the most restrictive taken at the national level against unvaccinated people.
Only children and people who cannot receive vaccines for medical reasons will be exempt.
This surge in cases comes as Germany has been in political limbo since the parliamentary elections in September.
Covid-19: cases at the highest in Germany AFP
The parties currently negotiating a new coalition to form a government by early December have so far ruled out compulsory vaccination and the introduction of new lockdowns, at least for those vaccinated.
© 2021 AFP