China News Service, Hangzhou, December 16 (Guo Qiyu) In the face of a sudden epidemic, nucleic acid testing has become an important line of defense to stop the spread of the epidemic. So far, Zhejiang has carried out a total of 12.966 million nucleic acid tests.

Behind such a huge workload is the "race" between the staff and the new crown virus.

  Weng Yuesong, a member of the Peasants and Workers Party and a laboratory physician at Ningbo No. 1 Hospital in Zhejiang Province, used words to record the bits and pieces behind nucleic acid testing during his busy schedule, and wrote down a handwritten note "The days when we raced against nucleic acid screening!"

", between the lines are serious and meticulous work, care for family colleagues, and longing for victory in the fight against the epidemic.

The laboratory ushered in the big test

  On December 6, the sudden epidemic affected the hearts of the people in the entire city, and our laboratory truly ushered in the test of its testing capabilities.

On the way to work, when I heard the Zhenhai epidemic on the radio, I had already calculated the work schedule for the day in my mind.

  After arriving at the laboratory, I have received a notice of "receiving nucleic acid test samples from Zhenhai Jiaochuan", and the exact number is unclear.

I immediately set out to prepare for the preparation work. First, I vacated the room of the mass spectrometry room for sample reception; confirmed that the information system was unblocked and usable, and enabled the 2D area as the first wave of sample extraction and sample addition area; each biosafety cabinet required items were in place .

  Our reagent preparation team is in place first, and prepares 3,000 copies of extraction and amplification reagents. The record forms of various processes have also been printed and prepared.

The laboratory staff all actively joined the nucleic acid testing team, and those who did not participate in the nucleic acid testing also took the initiative to undertake related auxiliary work.

  At 14:34, the first batch of specimens arrived in the laboratory, with a total of 172 tubes. It was easier for everyone to complete.

As the sampling progress accelerated, a batch of specimens was sent every 2 hours or so, and the number of specimens in each batch was increasing.

We immediately used area 2B as the nucleic acid sample extraction and extraction area. The second group of samplers entered the warehouse and the two groups of people started work at the same time, which immediately improved efficiency.

  We received a total of 10 batches of samples that day, and when the last batch of samples arrived, it was already 0:45 in the morning.

When the last batch of reports came out, it was around 5 a.m. on the 7th.

  On the morning of December 7th, I probably only slept for two hours.

I kept getting phone calls and really couldn't sleep peacefully, so I just got up to work.

Colleagues are moving

  On December 8th, I have to mention our colleague Dai Yanping. She is the backbone of the report review team. She not only has to contact the supply of various consumables, but also install and debug the 10 new thermal cyclers in the laboratory.

She re-arranged and adjusted the space of the 3 districts that were already crowded, and installed these 10 machines to "settled down" in order to prepare for the next challenge of dealing with more specimens.

  Dai Yanping also keeps coming up with golden ideas. She has newly established a large-scale screening team for COVID-19 in LIS, which not only solves the problem of mixing with the daily specimens of this hospital, but also solves the need for each package code to be reviewed by LIS. The problem of manually adding items has further improved the review efficiency.

  On December 9th, the total number of external samples today is 9,204 tubes, plus the more than 3,000 tubes in this hospital, the daily inspection volume has reached 12,000 tubes.

Although the detection volume has hit a new high, it feels easier than a few days ago. The task was completed before 8pm.

This is due to the continuous optimization of the process, the more and more tacit cooperation of everyone, and the sufficient and adequate provision of auxiliary personnel.

  Siji is usually mainly engaged in scientific research. Facing the epidemic, he took the initiative to ask Ying to help. Chen Lan and Li Li, both of whom were pregnant, also took the initiative to help.

Taking into account the actual situation, several of them are responsible for the preparation of reagents and provide a steady stream of "ammunition" for the front line to ensure that there will be no delays in sample addition and extraction because the preparation of reagents cannot keep up.

Today Xu Ling had a sudden kidney stone and the pain was unbearable. He returned to work after the painkiller, which was moving.

Detection speed reaches its limit

  On December 10th, I don't know "what year is this eve?" I don't know "what year is this eve" on December 10th. I occasionally glanced at my phone to realize that today is the weekend.

My son called me and asked when I could go home. He said that he missed me. This kid would also say that he missed me. It's rare!

I told him to take care of his own study, and don't let us worry about helping us.

The boy agreed very simply, wondering if he can really manage himself.

  The first batch of samples arrived at 9:50 today, and the results will be available on the first board at about 11:30.

At that time, I didn’t pay attention to the time. At night, I suddenly realized that the time from receiving the specimen to the first plate reporting was only about 1 hour and 40 minutes. This means that the 12 minutes of extraction and 1 hour of amplification time are removed and used for sample sorting. , Shaking, transporting, adding samples, adding products, sealing, centrifugation, machine and analysis report, etc. The time is only 28 minutes.

This speed should be the limit, I am shocked by it myself.

  On December 12, the third round of nucleic acid screening conference in Zhenhai District began.

The first batch of specimens were delivered at 8:20 in the morning. The sample sorting group was busy in the most advanced field. The reagent and consumable preparation group was also preparing reagents and consumables for the sample extraction area at the same time, and the staff of the sample addition team was ready to enter the sample. Area.

  With the continuous delivery of specimens, the sample sorting team, sample addition team, and report review team are perfectly matched. There is almost no sample accumulation. Our entire nucleic acid testing team is like a machine, running efficiently and continuously.

By about 14:40, our team had received more than 11,300 tube samples, all of which had been sorted.

In addition to the samples from our hospital, more than 20,000 tubes of samples were tested that day.

  We are grateful to the logistical cleaning and security personnel who silently pay in the back. They deal with so many samples and waste every day, and they also fight side by side with us.

It is with so many people, regardless of their personal gains and losses, that we can win this battle against the virus.

I hope that the epidemic will end as soon as possible and draw a successful conclusion to 2021.

(Finish)