A girl rescued from the Rhine died after attempting resuscitation in the hospital.
The fire brigade and police confirmed the death of the 17-year-old on Thursday night.
Two other girls aged 13 and 14 were still missing after they too had stepped into the Rhine near Duisburg.
According to the fire brigade, the search for them was broken off at around 10 p.m. "because there was no longer any way to save the two of them alive".
The Bild newspaper had previously reported on it.
According to the fire brigade, several people were reported missing in the Rhine at around 5:50 p.m.
A larger number of people were on the bank, so 20 to 40 people were looked after on site.
When searching from the air, the 17-year-old was spotted in the water and rescued with a lifeboat.
The search for the other two missing girls continued downstream, around 160 workers, including fire and rescue services, as well as two helicopters and several divers were on duty.
About four hours later the search was canceled after dark.
A 29-year-old drowned last week
On Saturday, the body of a 29-year-old was discovered in the Rhine after a swimming accident.
The man had gone swimming in the Rhine the previous Wednesday in Rheinberg-Orsoy (Wesel district) and after a few meters called for help, the police said, citing witnesses.
He tried to save himself on the bank, but then sank and was no longer seen.
The body was finally found in the Waal near Nijmegen (Netherlands), several kilometers further northwest.
The river Waal is an arm of the Rhine that flows into the North Sea.