Pictures from Saturday's live broadcast showed how the singer Damiano David bent down with his face across the table.
This was interpreted by some as the singer snorting drugs.
When the speculation gained momentum, the band requested a drug test.
It has now been implemented and the result is negative, the EBU announces in a press release.
"No drug use occurred in the green room and we consider the matter closed," the EBU wrote in the statement.
The article is updated