The work "Game changer" was sold by the auction house Christies in London and the final price is the highest ever paid for a Banksy work.
The money goes in full to support British healthcare workers during the pandemic.
"Game changer" first appeared in a hospital in Southampton during the first wave of viruses last year.
The work depicts a boy holding a superhero doll in a nurse's uniform while the two classic superheroes Batman and Superman are sent to the trash.
The latest work by Banksy, whose identity is still unknown, which was sold was "Show me the Monet", which went under the hammer last autumn for SEK 87 million.