Main lot of this sale, a Defender 110 (estimate of 300,000 to 500,000 pounds sterling) famous for its action scenes in No Time To Die.
Proceeds from the sale will be donated to the British Red Cross.
One of the ten Defenders used during filming, it was also used for promotional activities before the film's release.
A Defender 110 V8 Bond Edition, created by SV Bespoke and inspired by the specification of the Defenders used in No Time To Die, will also go on sale (estimate £200,000-300,000).
Produced in only 300 copies, the Bond Edition version sports a “60 Years of Bond” logo on the dashboard.
This vehicle is UK specification, street legal and will be sold for the benefit of the conservation charity Tusk.
The Range Rover Sport SVR (estimated at £80,000-120,000) also featured in one of the film's chase scenes and was one of six models provided for filming.
Finally, named by the No Time To Die stunt team as the perfect racing vehicle, the Jaguar XF (estimated between £50,000 and £70,000) is one of two models that appear in the film's pre-credits.
They were used to chase James Bond (Daniel Craig) and Madeleine Swann (Léa Seydoux) through the narrow, winding streets of Matera, southern Italy.
Sale in two parts
This two-part charity sale, Sixty Years of James Bond, will include a total of 60 lots.
The online sale will be open for auction from September 15 to October 5, the date of James Bond Day.
35 lots covering the 25 James Bonds will be presented, including posters, props and costumes.
The Defender, Range Rover and Jaguar from the film are among 25 lots in the invitation-only auction organized by Christie's and EON Productions in London on September 28.
Fans and collectors around the world will be able to bid online through Christie's Live.
Jaguar F-Pace SVR Edition 1988: Very exclusive commemorative special edition
Land Rover Defender 130: The Adventure of Eight