The meeting, which took place at the Ak Bars Arena in Kazan, ended in a victory for the hosts - 1: 0.
The only goal in the match was scored by Ilya Samoshnikov.
Spartak played their first match in the RPL under the guidance of a new coach - Portuguese specialist Rui Vitoria.
Earlier it was reported that Zhemaletdinov's double helped Lokomotiv beat Arsenal in the first round of the RPL.